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pf-127 aldrich)/water gel solution  (Millipore)


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    Millipore pf-127 aldrich)/water gel solution
    A schematic overview of the procedures used to produce the data in B-D. C. elegans are washed from NGM culture plates into a 1.5 mL tube and washed 3 times to remove residual debris and bacteria and to enrich for young adult worms. Worms are then mixed with liquid <t>PF-127</t> and quickly added to the devices to allow for polymerization of the hydrogel. Initial experiments used 24-well devices with 3 slices, and later experiments used the gustatory microplate. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and then devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) C. elegans are attracted to either 100 mM or 200 mM NaCl, with a slightly higher CI when the cue was added to the top of the well. (C) Three-layer Stacks devices were separated after 1 hr. incubation, and worms in each slice were counted. Wild type (N2) worms are attracted to 200 mM NaCl and repelled by 10 mM quinine. tax-4(p678) worms show no response to NaCl and are attracted to quinine. (D) Chemotaxis indices of five chemosensory-defective knockout strains of C. elegans in response to 200 mM NaCl and 10 mM quinine. In each figure, dots represent the value for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. ***: p <= 0.001, ****: p <= 0.0001.
    Pf 127 Aldrich)/Water Gel Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pf-127+gel/pf127/bio_rxiv__2023__04__25__538347-99-6-8
    Average 90 stars, based on 1 article reviews
    pf-127 aldrich)/water gel solution - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "A high-throughput nematode sensory assay reveals an inhibitory effect of ivermectin on parasite gustation"

    Article Title: A high-throughput nematode sensory assay reveals an inhibitory effect of ivermectin on parasite gustation

    Journal: bioRxiv

    doi: 10.1101/2023.04.25.538347

    A schematic overview of the procedures used to produce the data in B-D. C. elegans are washed from NGM culture plates into a 1.5 mL tube and washed 3 times to remove residual debris and bacteria and to enrich for young adult worms. Worms are then mixed with liquid PF-127 and quickly added to the devices to allow for polymerization of the hydrogel. Initial experiments used 24-well devices with 3 slices, and later experiments used the gustatory microplate. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and then devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) C. elegans are attracted to either 100 mM or 200 mM NaCl, with a slightly higher CI when the cue was added to the top of the well. (C) Three-layer Stacks devices were separated after 1 hr. incubation, and worms in each slice were counted. Wild type (N2) worms are attracted to 200 mM NaCl and repelled by 10 mM quinine. tax-4(p678) worms show no response to NaCl and are attracted to quinine. (D) Chemotaxis indices of five chemosensory-defective knockout strains of C. elegans in response to 200 mM NaCl and 10 mM quinine. In each figure, dots represent the value for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. ***: p <= 0.001, ****: p <= 0.0001.
    Figure Legend Snippet: A schematic overview of the procedures used to produce the data in B-D. C. elegans are washed from NGM culture plates into a 1.5 mL tube and washed 3 times to remove residual debris and bacteria and to enrich for young adult worms. Worms are then mixed with liquid PF-127 and quickly added to the devices to allow for polymerization of the hydrogel. Initial experiments used 24-well devices with 3 slices, and later experiments used the gustatory microplate. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and then devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) C. elegans are attracted to either 100 mM or 200 mM NaCl, with a slightly higher CI when the cue was added to the top of the well. (C) Three-layer Stacks devices were separated after 1 hr. incubation, and worms in each slice were counted. Wild type (N2) worms are attracted to 200 mM NaCl and repelled by 10 mM quinine. tax-4(p678) worms show no response to NaCl and are attracted to quinine. (D) Chemotaxis indices of five chemosensory-defective knockout strains of C. elegans in response to 200 mM NaCl and 10 mM quinine. In each figure, dots represent the value for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. ***: p <= 0.001, ****: p <= 0.0001.

    Techniques Used: Incubation, Chemotaxis Assay, Knock-Out

    (A) A schematic overview of the procedures used to produce the data in B. L3s were extracted from mosquitoes and washed 3 times prior to being mixed with liquid PF-127 and added to devices to allow for polymerization. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) L3s from Brugia pahangi are attracted to FBS. Two methods for calculating chemotaxis index are shown. Dots represent the CI for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. *: p <= 0.05.
    Figure Legend Snippet: (A) A schematic overview of the procedures used to produce the data in B. L3s were extracted from mosquitoes and washed 3 times prior to being mixed with liquid PF-127 and added to devices to allow for polymerization. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) L3s from Brugia pahangi are attracted to FBS. Two methods for calculating chemotaxis index are shown. Dots represent the CI for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. *: p <= 0.05.

    Techniques Used: Incubation, Chemotaxis Assay

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    Image Search Results


    A schematic overview of the procedures used to produce the data in B-D. C. elegans are washed from NGM culture plates into a 1.5 mL tube and washed 3 times to remove residual debris and bacteria and to enrich for young adult worms. Worms are then mixed with liquid PF-127 and quickly added to the devices to allow for polymerization of the hydrogel. Initial experiments used 24-well devices with 3 slices, and later experiments used the gustatory microplate. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and then devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) C. elegans are attracted to either 100 mM or 200 mM NaCl, with a slightly higher CI when the cue was added to the top of the well. (C) Three-layer Stacks devices were separated after 1 hr. incubation, and worms in each slice were counted. Wild type (N2) worms are attracted to 200 mM NaCl and repelled by 10 mM quinine. tax-4(p678) worms show no response to NaCl and are attracted to quinine. (D) Chemotaxis indices of five chemosensory-defective knockout strains of C. elegans in response to 200 mM NaCl and 10 mM quinine. In each figure, dots represent the value for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. ***: p <= 0.001, ****: p <= 0.0001.

    Journal: bioRxiv

    Article Title: A high-throughput nematode sensory assay reveals an inhibitory effect of ivermectin on parasite gustation

    doi: 10.1101/2023.04.25.538347

    Figure Lengend Snippet: A schematic overview of the procedures used to produce the data in B-D. C. elegans are washed from NGM culture plates into a 1.5 mL tube and washed 3 times to remove residual debris and bacteria and to enrich for young adult worms. Worms are then mixed with liquid PF-127 and quickly added to the devices to allow for polymerization of the hydrogel. Initial experiments used 24-well devices with 3 slices, and later experiments used the gustatory microplate. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and then devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) C. elegans are attracted to either 100 mM or 200 mM NaCl, with a slightly higher CI when the cue was added to the top of the well. (C) Three-layer Stacks devices were separated after 1 hr. incubation, and worms in each slice were counted. Wild type (N2) worms are attracted to 200 mM NaCl and repelled by 10 mM quinine. tax-4(p678) worms show no response to NaCl and are attracted to quinine. (D) Chemotaxis indices of five chemosensory-defective knockout strains of C. elegans in response to 200 mM NaCl and 10 mM quinine. In each figure, dots represent the value for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. ***: p <= 0.001, ****: p <= 0.0001.

    Article Snippet: In addition to this, a 30% w/w PF-127 (Sigma Aldrich)/water gel solution was created 2-3 days before assay date to allow the PF-127 to dissolve completely at 4°C.

    Techniques: Incubation, Chemotaxis Assay, Knock-Out

    (A) A schematic overview of the procedures used to produce the data in B. L3s were extracted from mosquitoes and washed 3 times prior to being mixed with liquid PF-127 and added to devices to allow for polymerization. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) L3s from Brugia pahangi are attracted to FBS. Two methods for calculating chemotaxis index are shown. Dots represent the CI for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. *: p <= 0.05.

    Journal: bioRxiv

    Article Title: A high-throughput nematode sensory assay reveals an inhibitory effect of ivermectin on parasite gustation

    doi: 10.1101/2023.04.25.538347

    Figure Lengend Snippet: (A) A schematic overview of the procedures used to produce the data in B. L3s were extracted from mosquitoes and washed 3 times prior to being mixed with liquid PF-127 and added to devices to allow for polymerization. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) L3s from Brugia pahangi are attracted to FBS. Two methods for calculating chemotaxis index are shown. Dots represent the CI for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. *: p <= 0.05.

    Article Snippet: In addition to this, a 30% w/w PF-127 (Sigma Aldrich)/water gel solution was created 2-3 days before assay date to allow the PF-127 to dissolve completely at 4°C.

    Techniques: Incubation, Chemotaxis Assay